Some Features of Base Pair Mismatch and Heterology Repair in Escherichia coli
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چکیده
منابع مشابه
Some Features of Base Pair Mismatch and Heterology Escherichia coli Repair in
We have used artificially constructed heteroallelic heteroduplex molecules of bacteriophage lambda DNA to transfect Escherichia coli, and E. coli mutants deficient in various functions involved in the adenine methylation-directed mismatch repair system, MutL, MutS, MutH, and lJvrD (MutU). Analysis of the allele content of single infective centers shows that this repair system often acts on seve...
متن کاملRepair of defined single base-pair mismatches in Escherichia coli.
Heteroduplexes with single base-pair mismatches of known sequence were prepared by annealing separated strands of bacteriophage lambda DNA and used to transfect Escherichia coli. Each of the eight possible single base-pair mismatches was constructed. Genetic analysis of the progeny phages obtained from transfected bacteria indicates that the E. coli mismatch repair system does not recognize (or...
متن کاملSaturation of DNA mismatch repair and error catastrophe by a base analogue in Escherichia coli.
Deoxyribosyl-dihydropyrimido[4,5-c][1,2]oxazin-7-one (dP) is a potent mutagenic deoxycytidine-derived base analogue capable of pairing with both A and G, thereby causing G. C --> A. T and A. T --> G. C transition mutations. We have found that the Escherichia coli DNA mismatch-repair system can protect cells against this mutagenic action. At a low dose, dP is much more mutagenic in mismatch-repa...
متن کاملChromosomal directionality of DNA mismatch repair in Escherichia coli.
Defects in DNA mismatch repair (MMR) result in elevated mutagenesis and in cancer predisposition. This disease burden arises because MMR is required to correct errors made in the copying of DNA. MMR is bidirectional at the level of DNA strand polarity as it operates equally well in the 5' to 3' and the 3' to 5' directions. However, the directionality of MMR with respect to the chromosome, which...
متن کاملThe Escherichia coli methyl-directed mismatch repair system repairs base pairs containing oxidative lesions.
A major role of the methyl-directed mismatch repair (MMR) system of Escherichia coli is to repair postreplicative errors. In this report, we provide evidence that MMR also acts on oxidized DNA, preventing mutagenesis. When cells deficient in MMR are grown anaerobically, spontaneous mutation frequencies are reduced compared with those of the same cells grown aerobically. In addition, we show tha...
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ژورنال
عنوان ژورنال: Genetics
سال: 1987
ISSN: 1943-2631
DOI: 10.1093/genetics/117.3.381